Journal: Critical reviews in clinical laboratory sciences

Article Title: Epigenetic biomarkers: Current strategies and future challenges for their use in the clinical laboratory

doi: 10.1080/10408363.2017.1410520

Figure Lengend Snippet: Commercial kits and main characteristics of six kits designed for cfDNA isolation and purification from biofluids.

Article Snippet: For example, the QIAsymphony DSP Circulating DNA Kit (Qiagen, Hilden, Germany) is intended for in vitro diagnostic use, and it allows fully automated and simultaneous purification of human cfDNA from 2 to 4 ml of human plasma and urine, but this kit requires the QIAsymphony SP instrument (Qiagen, Hilden, Germany). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Kit name Manufacturer Additional and optional steps Advantages Disadvantages Protocol time QIAamp® circulating nucleic acid kit Qiagen Proteinase K digestion treatment Recommended DNase I treatment for RNA downstream studies Allows purification and concentration of free-circulating DNA, RNA, miRNA, and viral nucleic acids from human plasma, serum, urine, or other cell-free body fluids (4–8 ml) exist in format DSP for IVD use Requires 4ml of plasma 3 h NucleoSpin® Plasma XS kit Macherey-Nagel Proteinase K digestion treatment Allows nucleic acid purification and concentration from serum, plasma, and bronchial lavage (up to 240 μl), also it allows to purify DNA from buccal swabs and dried blood spots specially designed purification column allowing elution volumes as low as 5 μl - 30min-1h FitAmp™ plasma/serum DNA isolation kit Epigentek Proteinase K digestion treatment Allows DNA isolation from plasma, serum and other body fluids (500 μl) Low efficiencies compared with other kits [ 39 ] 15-30 min QIAamp DNA blood mini kit Qiagen Protease or proteinase K digestion treatment Allows nucleic acid purification from serum, plasma and urine (from 200 μl), also it allows to purify DNA from buccal swabs and dried blood spots exist in format DSP for IVD use - 2-3 h Maxwell 16 blood DNA kit Promega Allows nucleic acid purification from 500 μl.

Techniques: Isolation, Purification, Concentration Assay, Nucleic Acid Purification, DNA Extraction, DNA Purification, In Vitro, Diagnostic Assay